A nukleotidok és az aktinkötő fehérjék szerepe az aktin funkcionális konformációváltozásaiban

Abstract

Actin is one of the main component in eukaryote cells which plays significant role in many cellular processes, like force-generation, maintenance of the shape of cells, celldivision cycle, and transport processes. Many structural investigations showed that the monomer actin (G-actin) consists of four subdomains, the internal structure of subdomains are stabilized by nucleotide, either ATP or ADP and a divalent cation (calcium or magnesium). The structure of subdomains is dynamic, as derived by several biophysical and structural studies; the subdomain might have different motional states depending on the bound cations and nucleotides. Site-specific cross-linking among F-actin monomers inhibits the motion and force generation in myosin. The ability of G-actin to reversible polymerize into long filaments is essential for many cellular functions.